recombinant mouse cd4 protein  (Sino Biological)

Journal: bioRxiv

Article Title: Systemic Translocation of S. aureus Drives Anti-CD4 Autoimmunity in Treated HIV Infection

doi: 10.1101/2025.08.04.668434

Figure Lengend Snippet: (A) Z-scored plasma levels of 87 autoantibodies in controls (n = 32), untreated PWH (n = 32), and ART-suppressed PWH (n = 53). (B) Anti-CD4 and anti-prothrombin IgG levels. (C) Anti-CD8 IgG levels. (D) Correlation between anti-CD4 IgG levels and CD4+ T cell counts. ANOVA and Spearman correlation tests were applied.

Article Snippet: The 96-well plate was coated with 1 μg/100 μL/well of recombinant mouse CD4 protein (Sino Biological, Wayne, PA) at 4°C overnight.

Techniques: Clinical Proteomics

Journal: bioRxiv

Article Title: Systemic Translocation of S. aureus Drives Anti-CD4 Autoimmunity in Treated HIV Infection

doi: 10.1101/2025.08.04.668434

Figure Lengend Snippet: Association of S. aureus serological and cellular markers with anti-CD4 IgG in PWH on ART. (A) Positive correlation between plasma anti-CD4 IgG and anti- S. aureus IgG levels. (B) Subgroup of HIV+/ART+ individuals shows elevated anti- S. aureus IgG. (C–D) B cell gene expression analysis reveals enriched immune pathways in PWH vs. controls. Spearman correlation and ANOVA were applied; P < 0.05.

Article Snippet: The 96-well plate was coated with 1 μg/100 μL/well of recombinant mouse CD4 protein (Sino Biological, Wayne, PA) at 4°C overnight.

Techniques: Clinical Proteomics, Gene Expression

Journal: bioRxiv

Article Title: Systemic Translocation of S. aureus Drives Anti-CD4 Autoimmunity in Treated HIV Infection

doi: 10.1101/2025.08.04.668434

Figure Lengend Snippet: C57BL/6J mice received i.p. injections of PBS, S. aureus PGN, or B. subtilis PGN twice weekly for 12 weeks (n = 8/group). (A–C) Serum levels of anti-CD4 IgG, total IgG, and anti-CD4 IgG subclasses. (D–E) Gating strategy and CD4+ T cell percentages in mesenteric lymph nodes, spleen, and blood. Student’s t-test and ANOVA were applied. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.

Article Snippet: The 96-well plate was coated with 1 μg/100 μL/well of recombinant mouse CD4 protein (Sino Biological, Wayne, PA) at 4°C overnight.

Techniques:

Journal: bioRxiv

Article Title: Systemic Translocation of S. aureus Drives Anti-CD4 Autoimmunity in Treated HIV Infection

doi: 10.1101/2025.08.04.668434

Figure Lengend Snippet: (A) Annexin V binding on IgG+CD4+ vs. IgG–CD4+ T cells. (B–D) Frequencies of IgG+CD4+ T cells in mesenteric lymph nodes, spleen, and blood. (E–G) Annexin V binding on IgG+CD4+ and IgG−CD4+ T cells in the same tissues. Student’s t-test and one-way ANOVA were applied.

Article Snippet: The 96-well plate was coated with 1 μg/100 μL/well of recombinant mouse CD4 protein (Sino Biological, Wayne, PA) at 4°C overnight.

Techniques: Binding Assay

Journal: Theranostics

Article Title: Controlled intracellular aggregation of magnetic particles improves permeation and retention for magnetic hyperthermia promotion and immune activation

doi: 10.7150/thno.80821

Figure Lengend Snippet: Detection of the intracellular aggregation induced permeation, retention and immune activation in vivo. (A) Schematic illustration of tumoral administration and possible immune activation process of different MNPs. (B) Permeation and retention of MNPs in tumor sections by DAB-enhanced Prussian staining in different groups. Scale bars: 1 mm. (C) Retention area (mm 2 ) and (D) permeation depth (mm) of varied MNPs in tumor sections, calculated from the images of Figure B. Immunohistochemical staining and positive cell percentages of CD80 (E and F), Mrc1 (G and H) and CD4 (I and J) in tumors treated with different MNPs (400 ×). Scale bars: 100, 25, and 10 μm, respectively. *** P < 0.001.

Article Snippet: Primary antibodies against CD80, Mrc1, and CD4 were obtained from Boster (China).

Techniques: Activation Assay, In Vivo, Staining, Immunohistochemical staining